Ontology highlight
ABSTRACT:
SUBMITTER: Van Lanen SG
PROVIDER: S-EPMC2529154 | biostudies-literature | 2007 Oct
REPOSITORIES: biostudies-literature
Van Lanen Steven G SG Oh Tae-Jin TJ Liu Wen W Wendt-Pienkowski Evelyn E Shen Ben B
Journal of the American Chemical Society 20071006 43
The biosynthetic gene cluster for the enediyne antitumor antibiotic maduropeptin (MDP) from Actinomadura madurae ATCC 39144 was cloned and sequenced. Cloning of the mdp gene cluster was confirmed by heterologous complementation of enediyne polyketide synthase (PKS) mutants from the C-1027 producer Streptomyces globisporus and the neocarzinostatin producer Streptomyces carzinostaticus using the MDP enediyne PKS and associated genes. Furthermore, MDP was produced, and its apoprotein was isolated a ...[more]