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ABSTRACT:
SUBMITTER: Dvinskikh S
PROVIDER: S-EPMC2529225 | biostudies-literature | 2006 May
REPOSITORIES: biostudies-literature
Dvinskikh Sergey S Dürr Ulrich U Yamamoto Kazutoshi K Ramamoorthy Ayyalusamy A
Journal of the American Chemical Society 20060501 19
Bicelles are increasingly being used as membrane mimicking systems in NMR experiments to investigate the structure of membrane proteins. In this study, we demonstrate the effectiveness of a 2D solid-state NMR approach that can be used to measure the structural constraints, such as heteronuclear dipolar couplings between 1H, 13C, and 31P nuclei, in bicelles without the need for isotopic enrichment. This method does not require a high radio frequency power unlike the presently used rotating-frame ...[more]