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Crystallization and preliminary X-ray crystallographic study of flavoredoxin from Desulfovibrio vulgaris Miyazaki F.

ABSTRACT: Flavoredoxin from Desulfovibrio vulgaris Miyazaki F has been overexpressed, purified and crystallized using the sitting-drop vapour-diffusion method with 10%(w/v) PEG 8000, 0.2 M zinc acetate and 100 mM MES pH 6.0. The diffraction pattern of the crystal extended to 1.05 A resolution under cryogenic conditions. The space group was determined to be P3(1)21, with unit-cell parameters a = b = 53.35, c = 116.22 A. Phase determination was carried out by the SAD method using methylmercuric chloride.

SUBMITTER: Ueda Y

PROVIDER: S-EPMC2531261 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature

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Crystallization and preliminary X-ray crystallographic study of flavoredoxin from Desulfovibrio vulgaris Miyazaki F.

Ueda Yasufumi Y Shibata Naoki N Takeuchi Daisuke D Kitamura Masaya M Higuchi Yoshiki Y

Acta crystallographica. Section F, Structural biology and crystallization communications 20080820 Pt 9

Flavoredoxin from Desulfovibrio vulgaris Miyazaki F has been overexpressed, purified and crystallized using the sitting-drop vapour-diffusion method with 10%(w/v) PEG 8000, 0.2 M zinc acetate and 100 mM MES pH 6.0. The diffraction pattern of the crystal extended to 1.05 A resolution under cryogenic conditions. The space group was determined to be P3(1)21, with unit-cell parameters a = b = 53.35, c = 116.22 A. Phase determination was carried out by the SAD method using methylmercuric chloride. ...[more]

PMID: 18765921

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