Ontology highlight
ABSTRACT:
SUBMITTER: Griffin TJ
PROVIDER: S-EPMC2533114 | biostudies-literature | 2007 Nov
REPOSITORIES: biostudies-literature
Griffin Timothy J TJ Xie Hongwei H Bandhakavi Sricharan S Popko Jonathan J Mohan Archana A Carlis John V JV Higgins LeeAnn L
Journal of proteome research 20070929 11
For proteomic analysis using tandem mass spectrometry, linear ion trap instruments provide unsurpassed sensitivity but unreliably detect low mass peptide fragments, precluding their use with iTRAQ reagent-labeled samples. Although the popular LTQ linear ion trap supports analyzing iTRAQ reagent-labeled peptides via pulsed Q dissociation, PQD, its effectiveness remains questionable. Using a standard mixture, we found careful tuning of relative collision energy necessary for fragmenting iTRAQ reag ...[more]