Screening for EphB signaling effectors using SILAC with a linear ion trap-orbitrap mass spectrometer.
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ABSTRACT:
SUBMITTER: Zhang G
PROVIDER: S-EPMC2673988 | biostudies-literature | 2008 Nov
REPOSITORIES: biostudies-literature
Publications
Screening for EphB signaling effectors using SILAC with a linear ion trap-orbitrap mass spectrometer.
Zhang Guoan G Fenyö David D Neubert Thomas A TA
Journal of proteome research 20080925 11
Erythropoietin-producing hepatocellular carcinoma (Eph) receptors play important roles in development, neural plasticity, and cancer. We used an Orbitrap mass spectrometer and stable isotope labeling by amino acids in cell culture (SILAC) to identify and quantify 204 proteins with significantly changed abundance in antiphosphotyrosine immunoprecipitates after ephrinB1-Fc stimulation. More than half of all known effectors downstream of EphB receptors were identified in this study, as well as nume ...[more]
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