Ontology highlight
ABSTRACT:
SUBMITTER: Brousseau LC
PROVIDER: S-EPMC2533726 | biostudies-literature | 2006 Sep
REPOSITORIES: biostudies-literature
Journal of the American Chemical Society 20060901 35
Here we present a novel assay that eliminates fluorescent labels and enables "digital detection" of single-molecule DNA hybridization in complex matrixes with greatly simplified protocols. Electronic coupling of the binding state of a single oligonucleotide to the quantum dot (QD) of a single electron transistor (SET) affords direct observation of binding events in real-time via "molecular gating". The change of electrostatic charge associated with the molecular capture is used in lieu of a gate ...[more]