Enantioselective alpha-hydroxylation of 2-arylacetic acid derivatives and buspirone catalyzed by engineered cytochrome P450 BM-3.
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ABSTRACT:
SUBMITTER: Landwehr M
PROVIDER: S-EPMC2551755 | biostudies-literature | 2006 May
REPOSITORIES: biostudies-literature
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Enantioselective alpha-hydroxylation of 2-arylacetic acid derivatives and buspirone catalyzed by engineered cytochrome P450 BM-3.
Landwehr Marco M Hochrein Lisa L Otey Christopher R CR Kasrayan Alex A Bäckvall Jan-E JE Arnold Frances H FH
Journal of the American Chemical Society 20060501 18
Here we report that an engineered microbial cytochrome P450 BM-3 (CYP102A subfamily) efficiently catalyzes the alpha-hydroxylation of phenylacetic acid esters. This P450 BM-3 variant also produces the authentic human metabolite of buspirone, R-6-hydroxybuspirone, with 99.5% ee. ...[more]
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