Novel pathway compendium analysis elucidates mechanism of pro-angiogenic synthetic small molecule.

ABSTRACT:

Motivation

Computational techniques have been applied to experimental datasets to identify drug mode-of-action. A shortcoming of existing approaches is the requirement of large reference databases of compound expression profiles. Here, we developed a new pathway-based compendium analysis that couples multi-timepoint, controlled microarray data for a single compound with systems-based network analysis to elucidate drug mechanism more efficiently.

Results

We applied this approach to a transcriptional regulatory footprint of phthalimide neovascular factor 1 (PNF1)-a novel synthetic small molecule that exhibits significant in vitro endothelial potency-spanning 1-48 h post-supplementation in human micro-vascular endothelial cells (HMVEC) to comprehensively interrogate PNF1 effect

SUBMITTER: Wieghaus KA 

PROVIDER: S-EPMC2562016 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature