Ontology highlight
ABSTRACT:
SUBMITTER: Zhao Y
PROVIDER: S-EPMC2562868 | biostudies-literature | 2008 May
REPOSITORIES: biostudies-literature
Zhao Yunge Y Lyons Charles E CE Xiao Aizhen A Templeton Dennis J DJ Sang Qingxiang Amy QA Brew Keith K Hussaini Isa M IM
Biochemical and biophysical research communications 20080318 4
Previous reports showed that urokinase plasminogen activator (uPA) converts plasminogen to plasmin which then activates matrix metalloproteinases (MMPs). Here, we report that uPA directly cleaved pro-MMP-9 in a time-dependent manner at both C- and N-terminus and generated two gelatinolytic bands. uPA-activated-MMP-9 efficiently degraded fibronectin and blocked by uPA inhibitor B428 and recombinant tissue inhibitor of metalloproteinase-1 (TIMP-1). B428 inhibited basal and PMA-induced active MMP-9 ...[more]