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Quantum-dot-based nanosensor for RRE IIB RNA-Rev peptide interaction assay.

ABSTRACT: Rev is an important HIV-1 regulatory protein that binds the Rev responsive element (RRE) within the env gene of HIV-1 RNA genome; the binding of Rev to RRE is essential for the expression of the structural genes, gag-pol and env, and for HIV replication. Here we report a quantum-dot (QD)-based nanosensor that can be used in fluorescence resonance energy transfer (FRET) assays of RRE IIB RNA-Rev peptide interactions. In comparison with conventional fluorescent dye-based methods, this QD-based nanosensor offers the distinct advantages of not inhibiting the Rev-RRE interaction, high sensitivity, improved accuracy, and simultaneous FRET-related two-parameter detection. This QD-based nanosensor provides a new approach to study the effects of inhibitors upon Rev-RRE interaction, and it may have a wide applicability in the development of new drugs against HIV-1 infection.

SUBMITTER: Zhang CY

PROVIDER: S-EPMC2582526 | biostudies-literature | 2006 Apr

REPOSITORIES: biostudies-literature

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Quantum-dot-based nanosensor for RRE IIB RNA-Rev peptide interaction assay.

Zhang Chun-yang CY Johnson Lawrence W LW

Journal of the American Chemical Society 20060401 16

Rev is an important HIV-1 regulatory protein that binds the Rev responsive element (RRE) within the env gene of HIV-1 RNA genome; the binding of Rev to RRE is essential for the expression of the structural genes, gag-pol and env, and for HIV replication. Here we report a quantum-dot (QD)-based nanosensor that can be used in fluorescence resonance energy transfer (FRET) assays of RRE IIB RNA-Rev peptide interactions. In comparison with conventional fluorescent dye-based methods, this QD-based nan ...[more]

PMID: 16620087

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