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Cloning and characterization of alpha-methylacyl coenzyme A racemase from Gordonia polyisoprenivorans VH2.

ABSTRACT: The mcr gene of Gordonia polyisoprenivorans VH2 is not clustered with genes required for rubber degradation. Its disruption by insertion of a kanamycin resistance cassette impaired growth on methyl-branched isoprenoids but not on linear hydrocarbons. Intact mcr from this bacterium or from Nocardia farcinica IFM 10152 complemented the mutant. Reverse transcription analysis showed similar mcr(VH2) expression results during cultivation with poly(cis-1,4-isoprene) and propionate. Additional genes coding for a putative cytochrome P450 monooxygenase and a short-chain dehydrogenase/reductase involved in beta-oxidation and poly(cis-1,4-isoprene) degradation were also characterized.

SUBMITTER: Arenskotter Q

PROVIDER: S-EPMC2583472 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature

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Cloning and characterization of alpha-methylacyl coenzyme A racemase from Gordonia polyisoprenivorans VH2.

Arenskötter Quyen Q Heller Jens J Dietz David D Arenskötter Matthias M Steinbüchel Alexander A

Applied and environmental microbiology 20080926 22

The mcr gene of Gordonia polyisoprenivorans VH2 is not clustered with genes required for rubber degradation. Its disruption by insertion of a kanamycin resistance cassette impaired growth on methyl-branched isoprenoids but not on linear hydrocarbons. Intact mcr from this bacterium or from Nocardia farcinica IFM 10152 complemented the mutant. Reverse transcription analysis showed similar mcr(VH2) expression results during cultivation with poly(cis-1,4-isoprene) and propionate. Additional genes co ...[more]

PMID: 18820059

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Establishment of Tn5096-based transposon mutagenesis in Gordonia polyisoprenivorans.

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