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Expression and characterization of ?-methylacyl CoA racemase from Anisakis simplex larvae.

ABSTRACT: Larval excretory-secretory products of Anisakis simplex are known to cause allergic reactions in humans. A cDNA library of A. simplex 3rd-stage larvae (L3) was immunoscreened with polyclonal rabbit serum raised against A. simplex L3 excretory-secretory products to identify an antigen that elicits the immune response. One cDNA clone, designated as ?-methylacyl CoA racemase (Amacr) contained a 1,412 bp cDNA transcript with a single open reading frame that encoded 418 amino acids. A. simplex Amacr showed a high degree of homology compared to Amacr orthologs from other species. Amacr mRNA was highly and constitutively expressed regardless of temperature (10-40?) and time (24-48 hr). Immunohistochemical analysis revealed that Amacr was expressed mainly in the ventriculus of A. simplex larvae. The Amacr protein produced in large quantities from the ventriculus is probably responsible for many functions in the development and growth of A. simplex larvae.

SUBMITTER: Kim BJ 

PROVIDER: S-EPMC3375458 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature

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Expression and characterization of α-methylacyl CoA racemase from Anisakis simplex larvae.

Kim Bong Jin BJ   Kim Sun Mi SM   Cho Min Kyung MK   Yu Hak Sun HS   Lee Yong Seok YS   Cha Hee Jae HJ   Ock Meesun M  

The Korean journal of parasitology 20120524 2

Larval excretory-secretory products of Anisakis simplex are known to cause allergic reactions in humans. A cDNA library of A. simplex 3rd-stage larvae (L3) was immunoscreened with polyclonal rabbit serum raised against A. simplex L3 excretory-secretory products to identify an antigen that elicits the immune response. One cDNA clone, designated as α-methylacyl CoA racemase (Amacr) contained a 1,412 bp cDNA transcript with a single open reading frame that encoded 418 amino acids. A. simplex Amacr  ...[more]

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