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Mu2 adaptin facilitates but is not essential for synaptic vesicle recycling in Caenorhabditis elegans.


ABSTRACT: Synaptic vesicles must be recycled to sustain neurotransmission, in large part via clathrin-mediated endocytosis. Clathrin is recruited to endocytic sites on the plasma membrane by the AP2 adaptor complex. The medium subunit (micro2) of AP2 binds to cargo proteins and phosphatidylinositol-4 ,5-bisphosphate on the cell surface. Here, we characterize the apm-2 gene (also called dpy-23), which encodes the only micro2 subunit in the nematode Caenorhabditis elegans. APM-2 is highly expressed in the nervous system and is localized to synapses; yet specific loss of APM-2 in neurons does not affect locomotion. In apm-2 mutants, clathrin is mislocalized at synapses, and synaptic vesicle numbers and evoked responses are reduced to 60 and 65%, respectively. Collectively, these data suggest AP2 micro2 facilitates but is not essential for synaptic vesicle recycling.

SUBMITTER: Gu M 

PROVIDER: S-EPMC2592831 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature

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Mu2 adaptin facilitates but is not essential for synaptic vesicle recycling in Caenorhabditis elegans.

Gu Mingyu M   Schuske Kim K   Watanabe Shigeki S   Liu Qiang Q   Baum Paul P   Garriga Gian G   Jorgensen Erik M EM  

The Journal of cell biology 20081201 5


Synaptic vesicles must be recycled to sustain neurotransmission, in large part via clathrin-mediated endocytosis. Clathrin is recruited to endocytic sites on the plasma membrane by the AP2 adaptor complex. The medium subunit (micro2) of AP2 binds to cargo proteins and phosphatidylinositol-4 ,5-bisphosphate on the cell surface. Here, we characterize the apm-2 gene (also called dpy-23), which encodes the only micro2 subunit in the nematode Caenorhabditis elegans. APM-2 is highly expressed in the n  ...[more]

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