Unknown

Dataset Information

0

Long-term vector integration site analysis following retroviral mediated gene transfer to hematopoietic stem cells for the treatment of HIV infection.


ABSTRACT: We previously reported the efficacy of nonmyeloablative allogeneic transplantation in 2 HIV positive recipients, one of whom received retrovirus transduced hematopoietic stem cells to confer resistance to HIV. Here we report an assessment of retroviral integration sites (RISs) recovered out to 3 years post-transplantation. We identified 213 unique RISs from the patient's peripheral blood samples by linear amplification-mediated PCR (LAM-PCR). While vector integration patterns were similar to that previously reported, only 3.76% of RISs were common among early (up to 3 months) and late samples (beyond 1 year). Additionally, common integration sites were enriched among late samples (14.9% vs. 36.8%, respectively). Three RISs were found near or within known oncogenes, but 2 were limited to early timepoints. Interestingly, an integration site near the MDS1 gene was detected in long-term follow-up samples; however, the overall contribution of MDS1 integrated clone remained stably low during follow-up.

SUBMITTER: Hayakawa J 

PROVIDER: S-EPMC2615408 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature

altmetric image

Publications

Long-term vector integration site analysis following retroviral mediated gene transfer to hematopoietic stem cells for the treatment of HIV infection.

Hayakawa Jun J   Washington Kareem K   Uchida Naoya N   Phang Oswald O   Kang Elizabeth M EM   Hsieh Matthew M MM   Tisdale John F JF  

PloS one 20090116 1


We previously reported the efficacy of nonmyeloablative allogeneic transplantation in 2 HIV positive recipients, one of whom received retrovirus transduced hematopoietic stem cells to confer resistance to HIV. Here we report an assessment of retroviral integration sites (RISs) recovered out to 3 years post-transplantation. We identified 213 unique RISs from the patient's peripheral blood samples by linear amplification-mediated PCR (LAM-PCR). While vector integration patterns were similar to tha  ...[more]

Similar Datasets

| S-EPMC5053271 | biostudies-literature
| S-EPMC3616749 | biostudies-literature
| S-EPMC1895268 | biostudies-literature
| S-EPMC509299 | biostudies-literature
| S-EPMC4176367 | biostudies-literature
| S-EPMC4426797 | biostudies-other
| S-EPMC2991247 | biostudies-literature
| S-EPMC3510789 | biostudies-literature
| S-EPMC3129560 | biostudies-literature
| S-EPMC1801061 | biostudies-literature