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Synthesis and characterization of BODIPY-labeled colchicine.

ABSTRACT: Two BODIPY-labeled colchicine derivatives were synthesized and shown to bind to tubulin but only partially inhibit tubulin polymerization in the presence of GTP. Cytotoxicity studies were carried out in HeLa, HepG2, Raji and Vero cells. Apoptosis-inducing properties were determined by caspase 3/7 activity and flow cytometry and interactions between the derivatives and tubulin were verified by fluorescence microscopy of living cells.

SUBMITTER: Arnold LA

PROVIDER: S-EPMC2631555 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature

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Synthesis and characterization of BODIPY-labeled colchicine.

Arnold Leggy A LA Ranaivo Patricia P Guy R Kiplin RK

Bioorganic & medicinal chemistry letters 20080720 22

Two BODIPY-labeled colchicine derivatives were synthesized and shown to bind to tubulin but only partially inhibit tubulin polymerization in the presence of GTP. Cytotoxicity studies were carried out in HeLa, HepG2, Raji and Vero cells. Apoptosis-inducing properties were determined by caspase 3/7 activity and flow cytometry and interactions between the derivatives and tubulin were verified by fluorescence microscopy of living cells. ...[more]

PMID: 18684625

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