Ontology highlight
ABSTRACT:
SUBMITTER: Frank GA
PROVIDER: S-EPMC2632776 | biostudies-literature | 2008 Jul
REPOSITORIES: biostudies-literature
Frank Gabriel A GA Kipnis Yakov Y Smolensky Elena E Daube Shirley S SS Horovitz Amnon A Haran Gilad G
Bioconjugate chemistry 20080624 7
We describe the design of an optical switch in the chaperonin GroEL that is opened and closed by its ATP- and cochaperonin GroES-driven conformational changes. The switch, based on a fluorophore and a quencher, is engineered into the single-ring variant of the chaperone, and shows dramatic modulation of its fluorescent intensity in response to the transition of the protein between its allosteric states. It, therefore, forms a sensitive probe for the dynamics of the allosteric transitions of this ...[more]