Ontology highlight
ABSTRACT:
SUBMITTER: Bansal PK
PROVIDER: S-EPMC2635038 | biostudies-literature | 2009 Feb
REPOSITORIES: biostudies-literature
Bansal Parmil K PK Nourse Amanda A Abdulle Rashid R Kitagawa Katsumi K
The Journal of biological chemistry 20081210 6
The kinetochore, which consists of DNA sequence elements and structural proteins, is essential for high-fidelity chromosome transmission during cell division. In budding yeast, Sgt1 and Hsp90 help assemble the core kinetochore complex CBF3 by activating the CBF3 components Skp1 and Ctf13. In this study, we show that Sgt1 forms homodimers by performing in vitro and in vivo immunoprecipitation and analytical ultracentrifugation analyses. Analyses of the dimerization of Sgt1 deletion proteins showe ...[more]