Ontology highlight
ABSTRACT:
SUBMITTER: Kohoutova J
PROVIDER: S-EPMC2635868 | biostudies-literature | 2009 Feb
REPOSITORIES: biostudies-literature
Kohoutová J J Kutá Smatanová I I Brynda J J Lapkouski M M Revuelta J L JL Arellano J B JB Ettrich R R
Acta crystallographica. Section F, Structural biology and crystallization communications 20090107 Pt 2
Preliminary X-ray diffraction analysis of the extrinsic PsbP protein of photosystem II from spinach (Spinacia oleracea) was performed using N-terminally His-tagged recombinant PsbP protein overexpressed in Escherichia coli. Recombinant PsbP protein (thrombin-digested recombinant His-tagged PsbP) stored in bis-Tris buffer pH 6.00 was crystallized using the sitting-drop vapour-diffusion technique with PEG 550 MME as a precipitant and zinc sulfate as an additive. SDS-PAGE analysis of a dissolved cr ...[more]