Using NMR to distinguish viscosity effects from nonspecific protein binding under crowded conditions.
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ABSTRACT:
SUBMITTER: Li C
PROVIDER: S-EPMC2645536 | biostudies-literature | 2009 Feb
REPOSITORIES: biostudies-literature
Publications
Using NMR to distinguish viscosity effects from nonspecific protein binding under crowded conditions.
Li Conggang C Pielak Gary J GJ
Journal of the American Chemical Society 20090201 4
Conventional NMR approaches to detect weak protein binding and aggregation are hindered by the increased viscosity brought about by crowding. We describe a simple and reliable NMR method to distinguish viscosity effects from binding and aggregation under crowded conditions. ...[more]
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