Unknown

Dataset Information

0

ICP-MS-based multiplex profiling of glycoproteins using lectins conjugated to lanthanide-chelating polymers.


ABSTRACT: Lectins have been increasingly important in the study of glycoproteins. Here, we report a glycoprofiling method based on the covalent attachment of metal-chelating polymers to lectins for use in an ICP-MS-based assay. The labeled lectins are able to distinguish between glycoproteins covalently attached to a microtiter plate and their binding can be directly quantified by ICP-MS. Since each conjugate contains a different lanthanide, the assays can be conducted in a single or multiplex fashion, and may be readily elaborated to many different assay formats.

SUBMITTER: Leipold MD 

PROVIDER: S-EPMC2646003 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

ICP-MS-based multiplex profiling of glycoproteins using lectins conjugated to lanthanide-chelating polymers.

Leipold Michael D MD   Herrera Isaac I   Ornatsky Olga O   Baranov Vladimir V   Nitz Mark M  

Journal of proteome research 20090201 2


Lectins have been increasingly important in the study of glycoproteins. Here, we report a glycoprofiling method based on the covalent attachment of metal-chelating polymers to lectins for use in an ICP-MS-based assay. The labeled lectins are able to distinguish between glycoproteins covalently attached to a microtiter plate and their binding can be directly quantified by ICP-MS. Since each conjugate contains a different lanthanide, the assays can be conducted in a single or multiplex fashion, an  ...[more]

Similar Datasets

| S-EPMC3237748 | biostudies-literature
| S-EPMC5977404 | biostudies-literature
| S-EPMC2852895 | biostudies-literature
| S-EPMC8405517 | biostudies-literature
| S-EPMC3948941 | biostudies-literature
| S-EPMC7484035 | biostudies-literature
| S-EPMC5824709 | biostudies-literature
| S-EPMC3637892 | biostudies-other
| S-EPMC2759973 | biostudies-literature
| S-EPMC8340090 | biostudies-literature