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ICP-MS-based multiplex profiling of glycoproteins using lectins conjugated to lanthanide-chelating polymers.

ABSTRACT: Lectins have been increasingly important in the study of glycoproteins. Here, we report a glycoprofiling method based on the covalent attachment of metal-chelating polymers to lectins for use in an ICP-MS-based assay. The labeled lectins are able to distinguish between glycoproteins covalently attached to a microtiter plate and their binding can be directly quantified by ICP-MS. Since each conjugate contains a different lanthanide, the assays can be conducted in a single or multiplex fashion, and may be readily elaborated to many different assay formats.

SUBMITTER: Leipold MD

PROVIDER: S-EPMC2646003 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature

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ICP-MS-based multiplex profiling of glycoproteins using lectins conjugated to lanthanide-chelating polymers.

Leipold Michael D MD Herrera Isaac I Ornatsky Olga O Baranov Vladimir V Nitz Mark M

Journal of proteome research 20090201 2

Lectins have been increasingly important in the study of glycoproteins. Here, we report a glycoprofiling method based on the covalent attachment of metal-chelating polymers to lectins for use in an ICP-MS-based assay. The labeled lectins are able to distinguish between glycoproteins covalently attached to a microtiter plate and their binding can be directly quantified by ICP-MS. Since each conjugate contains a different lanthanide, the assays can be conducted in a single or multiplex fashion, an ...[more]

PMID: 19072657

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