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A novel chair-type G-quadruplex formed by a Bombyx mori telomeric sequence.


ABSTRACT: Recently, the human telomeric d[TAGGG(TTAGGG)(3)] sequence has been shown to form in K(+) solution an intramolecular (3+1) G-quadruplex structure, whose G-tetrad core contains three strands oriented in one direction and the fourth in the opposite direction. Here we present a study on the structure of the Bombyx mori telomeric d[TAGG(TTAGG)(3)] sequence, which differs from the human counterpart only by one G deletion in each repeat. We found that this sequence adopted multiple G-quadruplex structures in K(+) solution. We have favored a major G-quadruplex form by a judicious U-for-T substitution in the sequence and determined the folding topology of this form. We showed by NMR that this was a new chair-type intramolecular G-quadruplex which involved a two-layer antiparallel G-tetrad core and three edgewise loops. Our result highlights the effect of G-tract length on the folding topology of G-quadruplexes, but also poses the question of whether a similar chair-type G-quadruplex fold exists in the human telomeric sequences.

SUBMITTER: Amrane S 

PROVIDER: S-EPMC2647293 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature

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A novel chair-type G-quadruplex formed by a Bombyx mori telomeric sequence.

Amrane Samir S   Ang Rita Wan Lin RW   Tan Zhong Ming ZM   Li Chun C   Lim Joefina Kim Cheow JK   Lim Jocelyn Mei Wen JM   Lim Kah Wai KW   Phan Anh Tuân AT  

Nucleic acids research 20081222 3


Recently, the human telomeric d[TAGGG(TTAGGG)(3)] sequence has been shown to form in K(+) solution an intramolecular (3+1) G-quadruplex structure, whose G-tetrad core contains three strands oriented in one direction and the fourth in the opposite direction. Here we present a study on the structure of the Bombyx mori telomeric d[TAGG(TTAGG)(3)] sequence, which differs from the human counterpart only by one G deletion in each repeat. We found that this sequence adopted multiple G-quadruplex struct  ...[more]

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