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Transgenic mice with defined combinations of drug-inducible reprogramming factors.

ABSTRACT: Proviruses carrying drug-inducible Oct4, Sox2, Klf4 and c-Myc used to derive 'primary' induced pluripotent stem (iPS) cells were segregated through germline transmission, generating mice and cells carrying subsets of the reprogramming factors. Drug treatment produced 'secondary' iPS cells only when the missing factor was introduced. This approach creates a defined system for studying reprogramming mechanisms and allows screening of genetically homogeneous cells for compounds that can replace any transcription factor required for iPS cell derivation.

SUBMITTER: Markoulaki S

PROVIDER: S-EPMC2654270 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature

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Transgenic mice with defined combinations of drug-inducible reprogramming factors.

Markoulaki Styliani S Hanna Jacob J Beard Caroline C Carey Bryce W BW Cheng Albert W AW Lengner Christopher J CJ Dausman Jessica A JA Fu Dongdong D Gao Qing Q Wu Su S Cassady John P JP Jaenisch Rudolf R

Nature biotechnology 20090118 2

Proviruses carrying drug-inducible Oct4, Sox2, Klf4 and c-Myc used to derive 'primary' induced pluripotent stem (iPS) cells were segregated through germline transmission, generating mice and cells carrying subsets of the reprogramming factors. Drug treatment produced 'secondary' iPS cells only when the missing factor was introduced. This approach creates a defined system for studying reprogramming mechanisms and allows screening of genetically homogeneous cells for compounds that can replace any ...[more]

PMID: 19151700

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