Unknown

Dataset Information

0

Synthesis of programmable integrases.


ABSTRACT: Accurate modification of the 3 billion-base-pair human genome requires tools with exceptional sequence specificity. Here, we describe a general strategy for the design of enzymes that target a single site within the genome. We generated chimeric zinc finger recombinases with cooperative DNA-binding and catalytic specificities that integrate transgenes with >98% accuracy into the human genome. These modular recombinases can be reprogrammed: New combinations of zinc finger domains and serine recombinase catalytic domains generate novel enzymes with distinct substrate sequence specificities. Because of their accuracy and versatility, the recombinases/integrases reported in this work are suitable for a wide variety of applications in biological research, medicine, and biotechnology where accurate delivery of DNA is desired.

SUBMITTER: Gordley RM 

PROVIDER: S-EPMC2654808 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC6981159 | biostudies-literature
| S-EPMC5784857 | biostudies-literature
| S-EPMC3179663 | biostudies-literature
| S-EPMC7347301 | biostudies-literature
| S-EPMC10439120 | biostudies-literature
| S-EPMC6283847 | biostudies-other
| S-EPMC5161430 | biostudies-literature
| S-EPMC1986573 | biostudies-literature
| S-EPMC3947336 | biostudies-literature
| S-EPMC6168549 | biostudies-literature