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Expression, purification and preliminary X-ray crystallographic analysis of the chicken MHC class I molecule YF1*7.1.

ABSTRACT: YF1*7.1 is an allele of a polymorphic major histocompatibility complex (MHC) class I-like locus within the chicken Y gene complex. With the aim of understanding the possible role of the YF1*7.1 molecule in antigen presentation, the complex of YF1*7.1 heavy chain and beta(2)-microglobulin was reconstituted and purified without a peptide. Crystals diffracted synchrotron radiation to 1.32 A resolution and belonged to the monoclinic space group P2(1). The phase problem was solved by molecular replacement. A detailed examination of the structure may provide insight into the type of ligand that could be bound by the YF1*7.1 molecule.

SUBMITTER: Hee CS

PROVIDER: S-EPMC2664777 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature

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Expression, purification and preliminary X-ray crystallographic analysis of the chicken MHC class I molecule YF1*7.1.

Hee Chee Seng CS Gao Song S Miller Marcia M MM Goto Ronald M RM Ziegler Andreas A Daumke Oliver O Uchanska-Ziegler Barbara B

Acta crystallographica. Section F, Structural biology and crystallization communications 20090326 Pt 4

YF1*7.1 is an allele of a polymorphic major histocompatibility complex (MHC) class I-like locus within the chicken Y gene complex. With the aim of understanding the possible role of the YF1*7.1 molecule in antigen presentation, the complex of YF1*7.1 heavy chain and beta(2)-microglobulin was reconstituted and purified without a peptide. Crystals diffracted synchrotron radiation to 1.32 A resolution and belonged to the monoclinic space group P2(1). The phase problem was solved by molecular replac ...[more]

PMID: 19342797

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