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U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing.

ABSTRACT: U1 snRNP plays a crucial role in the 5' splice site recognition during splicing. Here we report the first example of naturally occurring U1-independent U2-type splicing in humans. The U1 components were not included in the pre-spliceosomal E complex formed on the human F1gamma (hF1gamma) intron 9 in vitro. Moreover, hF1gamma intron 9 was efficiently spliced even in U1-disrupted Xenopus oocytes as well as in U1-inactivated HeLa nuclear extracts. Finally, hF1gamma exon 9 skipping induced by an alternative splicing regulator Fox-1 was impaired when intron 9 was changed to the U1-dependent one. Our results suggest that U1-independent splicing contributes to the regulation of alternative splicing of a class of pre-mRNAs.

SUBMITTER: Fukumura K

PROVIDER: S-EPMC2665233 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature

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U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing.

Fukumura Kazuhiro K Taniguchi Ichiro I Sakamoto Hiroshi H Ohno Mutsuhito M Inoue Kunio K

Nucleic acids research 20090203 6

U1 snRNP plays a crucial role in the 5' splice site recognition during splicing. Here we report the first example of naturally occurring U1-independent U2-type splicing in humans. The U1 components were not included in the pre-spliceosomal E complex formed on the human F1gamma (hF1gamma) intron 9 in vitro. Moreover, hF1gamma intron 9 was efficiently spliced even in U1-disrupted Xenopus oocytes as well as in U1-inactivated HeLa nuclear extracts. Finally, hF1gamma exon 9 skipping induced by an alt ...[more]

PMID: 19190090

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