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FOXP3 interacts with hnRNPF to modulate pre-mRNA alternative splicing.

ABSTRACT: FOXP3 promotes the development and function of regulatory T cells mainly through regulating the transcription of target genes. RNA alternative splicing has been implicated in a wide range of physiological and pathophysiological processes. We report here that FOXP3 associates with heterogeneous nuclear ribonucleoprotein (hnRNP) F through the exon 2-encoded region of FOXP3 and the second quasi-RNA recognition motif (qRRM) of hnRNPF. FOXP3 represses the ability of hnRNPF to bind to its target pre-mRNA and thus modulates RNA alternative splicing. Furthermore, overexpression of mouse hnRNPF in in vitro-differentiated regulatory T cells (Tregs) reduced their suppressive function. Thus, our studies identify a novel mechanism by which FOXP3 regulates mRNA alternative splicing to modulate the function of regulatory T cells.

SUBMITTER: Du J

PROVIDER: S-EPMC6028976 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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FOXP3 interacts with hnRNPF to modulate pre-mRNA alternative splicing.

Du Jianguang J Wang Qun Q Ziegler Steven F SF Zhou Baohua B

The Journal of biological chemistry 20180517 26

FOXP3 promotes the development and function of regulatory T cells mainly through regulating the transcription of target genes. RNA alternative splicing has been implicated in a wide range of physiological and pathophysiological processes. We report here that FOXP3 associates with heterogeneous nuclear ribonucleoprotein (hnRNP) F through the exon 2-encoded region of FOXP3 and the second quasi-RNA recognition motif (qRRM) of hnRNPF. FOXP3 represses the ability of hnRNPF to bind to its target pre-m ...[more]

PMID: 29773655

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