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Genetic analysis of Drosophila melanogaster susceptibility to intestinal Vibrio cholerae infection.

ABSTRACT: We previously demonstrated that Vibrio cholerae is able to colonize the intestine of the fly to produce a lethal infection. Here we present the results of a genetic screen undertaken to identify factors that alter susceptibility of the fly to intestinal V. cholerae infection. In this model of infection, the Eiger/Wengen signalling pathway protects the fly against infection. Furthermore, mutations within the IMD signalling pathway increase resistance to intestinal V. cholerae infection and increase programmed cell death within the intestinal epithelium during infection. We propose that programmed cell death protects the intestinal epithelium against V. cholerae infection and therefore that the fly may serve as a useful model in which to study modulation of intestinal epithelial cell survival by commensal and pathogenic intestinal bacteria as well as the pathological processes leading to erosion of the intestinal epithelium and intestinal malignancy.

SUBMITTER: Berkey CD

PROVIDER: S-EPMC2666625 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature

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Genetic analysis of Drosophila melanogaster susceptibility to intestinal Vibrio cholerae infection.

Berkey Cristin D CD Blow Nathan N Watnick Paula I PI

Cellular microbiology 20081127 3

We previously demonstrated that Vibrio cholerae is able to colonize the intestine of the fly to produce a lethal infection. Here we present the results of a genetic screen undertaken to identify factors that alter susceptibility of the fly to intestinal V. cholerae infection. In this model of infection, the Eiger/Wengen signalling pathway protects the fly against infection. Furthermore, mutations within the IMD signalling pathway increase resistance to intestinal V. cholerae infection and increa ...[more]

PMID: 19046341

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