Efficient escape from endosomes determines the superior efficiency of multicomponent lipoplexes.
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ABSTRACT:
SUBMITTER: Caracciolo G
PROVIDER: S-EPMC2673460 | biostudies-literature | 2009 Apr
REPOSITORIES: biostudies-literature
Publications
Efficient escape from endosomes determines the superior efficiency of multicomponent lipoplexes.
Caracciolo Giulio G Caminiti Ruggero R Digman Michelle A MA Gratton Enrico E Sanchez Susana S
The journal of physical chemistry. B 20090401 15
Designer multicomponent lipoplexes have recently emerged as especially promising transfection candidates, since they are from 10 to 100 times more efficient than binary complexes usually employed for gene delivery purposes. Here, we show, for the first time, that after internalization binary complexes of lower transfection potency remain in compact perinuclear endosomes, while multicomponent systems have intrinsic endosomal rupture properties that allow plasmid DNA to escape from endosomes with ...[more]
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