Unknown

Dataset Information

0

Expression, purification, crystallization and preliminary crystallographic analysis of laminin-binding protein (Lmb) from Streptococcus agalactiae.

ABSTRACT: Laminin-binding protein (Lmb), a surface-exposed lipoprotein from Streptococcus agalactiae (group B streptococcus), mediates attachment to human laminin and plays a crucial role in the adhesion/invasion of eukaryotic host cells. However, the structural basis of laminin binding still remains unclear. In the context of detailed structural analysis, the lmb gene has been cloned, expressed in Escherichia coli, purified and crystallized. The crystals diffracted to a resolution of 2.5 A and belonged to the monoclinic space group P2(1), with unit-cell parameters a = 56.63, b = 70.60, c = 75.37 A, beta = 96.77 degrees .

SUBMITTER: Ragunathan P 

PROVIDER: S-EPMC2675593 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Expression, purification, crystallization and preliminary crystallographic analysis of laminin-binding protein (Lmb) from Streptococcus agalactiae.

Ragunathan Preethi P   Spellerberg Barbara B   Ponnuraj Karthe K  

Acta crystallographica. Section F, Structural biology and crystallization communications 20090424 Pt 5

Laminin-binding protein (Lmb), a surface-exposed lipoprotein from Streptococcus agalactiae (group B streptococcus), mediates attachment to human laminin and plays a crucial role in the adhesion/invasion of eukaryotic host cells. However, the structural basis of laminin binding still remains unclear. In the context of detailed structural analysis, the lmb gene has been cloned, expressed in Escherichia coli, purified and crystallized. The crystals diffracted to a resolution of 2.5 A and belonged t  ...[more]

Similar Datasets

Unknown Purification, crystallization and preliminary crystallographic analysis of Streptococcus pyogenes laminin-binding protein Lbp.
| S-EPMC2374178 | biostudies-literature
Unknown Metal binding is critical for the folding and function of laminin binding protein, Lmb of Streptococcus agalactiae.
| S-EPMC3691195 | biostudies-literature
Unknown Purification, crystallization and preliminary crystallographic analysis of SMU.1108c protein from Streptococcus mutans.
| S-EPMC3079977 | biostudies-literature
Unknown Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of glyceraldehyde-3-phosphate dehydrogenase from Streptococcus agalactiae NEM316.
| S-EPMC4089536 | biostudies-literature
Unknown Purification, crystallization and preliminary crystallographic analysis of the minor pilin FctB from Streptococcus pyogenes.
| S-EPMC2815686 | biostudies-literature
Unknown Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp6.
| S-EPMC4051540 | biostudies-literature
Unknown Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp1.
| S-EPMC3388920 | biostudies-literature
Unknown Overexpression, purification, crystallization and preliminary X-ray diffraction of the nisin resistance protein from Streptococcus agalactiae.
| S-EPMC4461328 | biostudies-literature
Unknown Expression, purification, crystallization and preliminary X-ray diffraction studies of the human keratin 4-binding domain of serine-rich repeat protein 1 from Streptococcus agalactiae.
| S-EPMC3232145 | biostudies-literature
Unknown Purification, crystallization and preliminary crystallographic analysis of the adhesion domain of Epf from Streptococcus pyogenes.
| S-EPMC3388924 | biostudies-literature