Ontology highlight
ABSTRACT:
SUBMITTER: Groen AC
PROVIDER: S-EPMC2688555 | biostudies-literature | 2009 Jun
REPOSITORIES: biostudies-literature
Groen Aaron C AC Maresca Thomas J TJ Gatlin Jesse C JC Salmon Edward D ED Mitchison Timothy J TJ
Molecular biology of the cell 20090415 11
Distinct pathways from centrosomes and chromatin are thought to contribute in parallel to microtubule nucleation and stabilization during animal cell mitotic spindle assembly, but their full mechanisms are not known. We investigated the function of three proposed nucleation/stabilization factors, TPX2, gamma-tubulin and XMAP215, in chromatin-promoted assembly of anastral spindles in Xenopus laevis egg extract. In addition to conventional depletion-add back experiments, we tested whether factors ...[more]