Ontology highlight
ABSTRACT:
SUBMITTER: Alian A
PROVIDER: S-EPMC2688900 | biostudies-literature | 2009 May
REPOSITORIES: biostudies-literature
Alian Akram A Griner Sarah L SL Chiang Vicki V Tsiang Manuel M Jones Gregg G Birkus Gabriel G Geleziunas Romas R Leavitt Andrew D AD Stroud Robert M RM
Proceedings of the National Academy of Sciences of the United States of America 20090504 20
HIV-1 integration into the host cell genome is a multistep process catalyzed by the virally-encoded integrase (IN) protein. In view of the difficulty of obtaining a stable DNA-bound IN at high concentration as required for structure determination, we selected IN-DNA complexes that form disulfide linkages between 5'-thiolated DNA and several single mutations to cysteine around the catalytic site of IN. Mild reducing conditions allowed for selection of the most thermodynamically-stable disulfide-l ...[more]