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Nanoscale live-cell imaging using hopping probe ion conductance microscopy.

ABSTRACT: We describe hopping mode scanning ion conductance microscopy that allows noncontact imaging of the complex three-dimensional surfaces of live cells with resolution better than 20 nm. We tested the effectiveness of this technique by imaging networks of cultured rat hippocampal neurons and mechanosensory stereocilia of mouse cochlear hair cells. The technique allowed examination of nanoscale phenomena on the surface of live cells under physiological conditions.

SUBMITTER: Novak P

PROVIDER: S-EPMC2702483 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature

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Nanoscale live-cell imaging using hopping probe ion conductance microscopy.

Novak Pavel P Li Chao C Shevchuk Andrew I AI Stepanyan Ruben R Caldwell Matthew M Hughes Simon S Smart Trevor G TG Gorelik Julia J Ostanin Victor P VP Lab Max J MJ Moss Guy W J GW Frolenkov Gregory I GI Klenerman David D Korchev Yuri E YE

Nature methods 20090301 4

We describe hopping mode scanning ion conductance microscopy that allows noncontact imaging of the complex three-dimensional surfaces of live cells with resolution better than 20 nm. We tested the effectiveness of this technique by imaging networks of cultured rat hippocampal neurons and mechanosensory stereocilia of mouse cochlear hair cells. The technique allowed examination of nanoscale phenomena on the surface of live cells under physiological conditions. ...[more]

PMID: 19252505

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