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ABSTRACT:
SUBMITTER: Shroff H
PROVIDER: S-EPMC5225950 | biostudies-literature | 2008 May
REPOSITORIES: biostudies-literature
Shroff Hari H Galbraith Catherine G CG Galbraith James A JA Betzig Eric E
Nature methods 20080413 5
We demonstrate live-cell super-resolution imaging using photoactivated localization microscopy (PALM). The use of photon-tolerant cell lines in combination with the high resolution and molecular sensitivity of PALM permitted us to investigate the nanoscale dynamics within individual adhesion complexes (ACs) in living cells under physiological conditions for as long as 25 min, with half of the time spent collecting the PALM images at spatial resolutions down to approximately 60 nm and frame rates ...[more]