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Exercise-induced up-regulation of MMP-1 and IL-8 genes in endurance horses.


ABSTRACT: BACKGROUND: The stress response is a critical factor in the training of equine athletes; it is important for performance and for protection of the animal against physio-pathological disorders.In this study, the molecular mechanisms involved in the response to acute and strenuous exercise were investigated using peripheral blood mononuclear cells (PBMCs). RESULTS: Quantitative real-time PCR (qRT-PCR) was used to detect modifications in transcription levels of the genes for matrix metalloproteinase-1 (MMP-1) and interleukin 8 (IL-8), which were derived from previous genome-wide expression analysis. Significant up-regulation of these two genes was found in 10 horses that had completed a race of 90-120 km in a time-course experimental design. CONCLUSION: These results suggest that MMP-1 and IL-8 are both involved in the exercise-induced stress response, and this represents a starting point from which to understand the adaptive responses to this phenomenon.

SUBMITTER: Cappelli K 

PROVIDER: S-EPMC2705340 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature

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Exercise-induced up-regulation of MMP-1 and IL-8 genes in endurance horses.

Cappelli Katia K   Felicetti Michela M   Capomaccio Stefano S   Pieramati Camillo C   Silvestrelli Maurizio M   Verini-Supplizi Andrea A  

BMC physiology 20090624


<h4>Background</h4>The stress response is a critical factor in the training of equine athletes; it is important for performance and for protection of the animal against physio-pathological disorders.In this study, the molecular mechanisms involved in the response to acute and strenuous exercise were investigated using peripheral blood mononuclear cells (PBMCs).<h4>Results</h4>Quantitative real-time PCR (qRT-PCR) was used to detect modifications in transcription levels of the genes for matrix met  ...[more]

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