Ontology highlight
ABSTRACT:
SUBMITTER: du Plessis DJ
PROVIDER: S-EPMC2708877 | biostudies-literature | 2009 Jun
REPOSITORIES: biostudies-literature
du Plessis David J F DJ Berrelkamp Greetje G Nouwen Nico N Driessen Arnold J M AJ
The Journal of biological chemistry 20090414 23
The SecYEG translocon of Escherichia coli mediates the translocation of preproteins across the cytoplasmic membrane. Here, we have examined the role of the proposed lateral gate of the translocon in translocation. A dual cysteine cross-linking approach allowed the introduction of cross-linker arms of various lengths between adjoining aminoacyl positions of transmembrane segments 2b and 7 of the lateral gate. Oxidation and short spacer linkers that fix the gate in the closed state abolished prepr ...[more]