Ontology highlight
ABSTRACT:
SUBMITTER: Kedrov A
PROVIDER: S-EPMC3230372 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Kedrov Alexej A Kusters Ilja I Krasnikov Victor V VV Driessen Arnold J M AJ
The EMBO journal 20110906 21
The heterotrimeric SecYEG complex comprises a protein-conducting channel in the bacterial cytoplasmic membrane. SecYEG functions together with the motor protein SecA in preprotein translocation. Here, we have addressed the functional oligomeric state of SecYEG when actively engaged in preprotein translocation. We reconstituted functional SecYEG complexes labelled with fluorescent markers into giant unilamellar vesicles at a natively low density. Förster's resonance energy transfer and fluorescen ...[more]