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Synthesis and characterization of stable fluorocarbon nanostructures as drug delivery vehicles for cytolytic peptides.

ABSTRACT: The therapeutic potential of cytolytic peptides is plagued by nonspecificity and enzymatic degradation. We report the first stable incorporation of melittin (a 26 amino acid amphipathic peptide) into an outer lipid monolayer of perfluorocarbon nanoparticles. Melittin binds avidly to the nanoparticles (dissociation constant approximately 3.27 nM) and retains its pore-forming activity after contact-mediated delivery to model bilayer membrane (liposomes) thereby demonstrating the effectiveness of perfluorocarbon nanoparticles as unique nanocarriers for cytolytic peptides.

SUBMITTER: Soman NR

PROVIDER: S-EPMC2710241 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature

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Synthesis and characterization of stable fluorocarbon nanostructures as drug delivery vehicles for cytolytic peptides.

Soman Neelesh R NR Lanza Gregory M GM Heuser John M JM Schlesinger Paul H PH Wickline Samuel A SA

Nano letters 20080227 4

The therapeutic potential of cytolytic peptides is plagued by nonspecificity and enzymatic degradation. We report the first stable incorporation of melittin (a 26 amino acid amphipathic peptide) into an outer lipid monolayer of perfluorocarbon nanoparticles. Melittin binds avidly to the nanoparticles (dissociation constant approximately 3.27 nM) and retains its pore-forming activity after contact-mediated delivery to model bilayer membrane (liposomes) thereby demonstrating the effectiveness of p ...[more]

PMID: 18302330

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