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Proteomic analysis of rat brain mitochondria following exposure to dopamine quinone: implications for Parkinson disease.

ABSTRACT: Oxidative stress and mitochondrial dysfunction have been linked to dopaminergic neuron degeneration in Parkinson disease. We have previously shown that dopamine oxidation leads to selective dopaminergic terminal degeneration in vivo and alters mitochondrial function in vitro. In this study, we utilized 2-D difference in-gel electrophoresis to assess changes in the mitochondrial proteome following in vitro exposure to reactive dopamine quinone. A subset of proteins exhibit decreased fluorescence labeling following dopamine oxidation, suggesting a rapid loss of specific proteins. Amongst these proteins are mitochondrial creatine kinase, mitofilin, mortalin, the 75 kDa subunit of NADH dehydrogenase, and superoxide dismutase 2. Western blot analyses for mitochondrial creatine kinase and mitofilin confirmed significant losses in isolated brain mitochondria exposed to dopamine quinone and PC12 cells exposed to dopamine. These results suggest that specific mitochondrial proteins are uniquely susceptible to changes in abundance following dopamine oxidation, and carry implications for mitochondrial stability in Parkinson disease neurodegeneration.

SUBMITTER: Van Laar VS

PROVIDER: S-EPMC2713672 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature

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Proteomic analysis of rat brain mitochondria following exposure to dopamine quinone: implications for Parkinson disease.

Van Laar Victor S VS Dukes April A AA Cascio Michael M Hastings Teresa G TG

Neurobiology of disease 20071128 3

Oxidative stress and mitochondrial dysfunction have been linked to dopaminergic neuron degeneration in Parkinson disease. We have previously shown that dopamine oxidation leads to selective dopaminergic terminal degeneration in vivo and alters mitochondrial function in vitro. In this study, we utilized 2-D difference in-gel electrophoresis to assess changes in the mitochondrial proteome following in vitro exposure to reactive dopamine quinone. A subset of proteins exhibit decreased fluorescence ...[more]

PMID: 18226537

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Proteomic analysis of rat brain mitochondria following exposure to dopamine quinone: implications for Parkinson disease.

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Proteomic analysis of rat brain mitochondria following exposure to dopamine quinone: implications for Parkinson disease.

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