The twin spot generator for differential Drosophila lineage analysis.
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ABSTRACT:
SUBMITTER: Griffin R
PROVIDER: S-EPMC2720837 | biostudies-literature | 2009 Aug
REPOSITORIES: biostudies-literature
Publications
The twin spot generator for differential Drosophila lineage analysis.
Griffin Ruth R Sustar Anne A Bonvin Marianne M Binari Richard R del Valle Rodriguez Alberto A Hohl Amber M AM Bateman Jack R JR Villalta Christians C Heffern Elleard E Grunwald Didier D Bakal Chris C Desplan Claude C Schubiger Gerold G Wu C-ting CT Perrimon Norbert N
Nature methods 20090726 8
In Drosophila melanogaster, widely used mitotic recombination-based strategies generate mosaic flies with positive readout for only one daughter cell after division. To differentially label both daughter cells, we developed the twin spot generator (TSG) technique, which through mitotic recombination generates green and red twin spots that are detectable after the first cell division as single cells. We propose wide applications of TSG to lineage and genetic mosaic studies. ...[more]
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