Ontology highlight
ABSTRACT:
SUBMITTER: Favaloro V
PROVIDER: S-EPMC2727622 | biostudies-literature | 2008 Jun
REPOSITORIES: biostudies-literature
Favaloro Vincenzo V Spasic Milan M Schwappach Blanche B Dobberstein Bernhard B
Journal of cell science 20080513 11
Tail-anchored (TA) proteins are characterised by a C-terminal transmembrane region that mediates post-translational insertion into the membrane of the endoplasmic reticulum (ER). We have investigated the requirements for membrane insertion of three TA proteins, RAMP4, Sec61beta and cytocrome b5. We show here that newly synthesised RAMP4 and Sec61beta can accumulate in a cytosolic, soluble complex with the ATPase Asna1 before insertion into ER-derived membranes. Membrane insertion of these TA pro ...[more]