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Parallax: high accuracy three-dimensional single molecule tracking using split images.

ABSTRACT: Three-dimensional (3D) tracking can provide valuable biological insights that are missing in conventional microscopy. Here we developed a single molecule 3D tracking microscopy technique, named Parallax, with high localization precision and temporal resolution. We demonstrated its capabilities by studying the 3D trafficking of glucose-transporter-4 containing vesicles in living adipocytes as well as the walking path of single myosin VI molecules along actin filaments.

SUBMITTER: Sun Y

PROVIDER: S-EPMC2728077 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature

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Parallax: high accuracy three-dimensional single molecule tracking using split images.

Sun Yujie Y McKenna Jennine Dawicki JD Murray John M JM Ostap E Michael EM Goldman Yale E YE

Nano letters 20090701 7

Three-dimensional (3D) tracking can provide valuable biological insights that are missing in conventional microscopy. Here we developed a single molecule 3D tracking microscopy technique, named Parallax, with high localization precision and temporal resolution. We demonstrated its capabilities by studying the 3D trafficking of glucose-transporter-4 containing vesicles in living adipocytes as well as the walking path of single myosin VI molecules along actin filaments. ...[more]

PMID: 19496608

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