Development of a CA125-mesothelin cell adhesion assay as a screening tool for biologics discovery.
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ABSTRACT:
SUBMITTER: Scholler N
PROVIDER: S-EPMC2734268 | biostudies-literature | 2007 Mar
REPOSITORIES: biostudies-literature
Publications
Development of a CA125-mesothelin cell adhesion assay as a screening tool for biologics discovery.
Scholler Nathalie N Garvik Barbara B Hayden-Ledbetter Martha M Kline Toni T Urban Nicole N
Cancer letters 20060504 1
Preventing peritoneal implantation of ovarian carcinoma cells could prolong patient remission and survival. CA125 is expressed on most ovarian cancer cells and was reported to be a ligand of mesothelin, a peritoneal protein. We developed a cell adhesion assay with CA125-expresser ovarian cancer cells and human mesothelin-transfected cells and we confirmed that CA125 and mesothelin mediate cell attachment. We also showed that this assay supplies a high-throughput screening system for reagents abl ...[more]
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