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The GTPase Rab3b/3c-positive recycling vesicles are involved in cross-presentation in dendritic cells.


ABSTRACT: Antigen cross-presentation in dendritic cells is a complex intracellular membrane transport process, but the underlying molecular mechanisms remain to be thoroughly investigated. In this study, we examined the effect of siRNA-mediated knockdown of 57 Rab GTPases, the key regulators of membrane trafficking, on antigen cross-presentation. Twelve Rab GTPases were identified to be associated with antigen cross-presentation, and Rab3b/3c was indicated to be colocalized with MHC class I molecules at perinuclear tubular structure. Tracing with fluorescence protein-tagged beta(2)-microglobulin demonstrated that the MHC class I molecules were internalized from the plasma membrane to Rab3b/3c-positive compartments, which were also colocalized with the internalized transferrin. Moreover, depletion of Rab3b/3c strongly reduced the fast phase recycling rate of transferrin receptors. Furthermore, the Rab3b/3c-positive compartments were colocalized with a fraction of Rab27a at a juxtaposition of phagosomes. Together, these data demonstrate that Rab3b/3c-positive recycling vesicles are involved in and may constitute one of the recycling compartments in exogenous antigen cross-presentation.

SUBMITTER: Zou L 

PROVIDER: S-EPMC2747199 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature

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The GTPase Rab3b/3c-positive recycling vesicles are involved in cross-presentation in dendritic cells.

Zou Liyun L   Zhou Jingran J   Zhang Jinyu J   Li Jingyi J   Liu Na N   Chai Linlin L   Li Na N   Liu Ting T   Li Liqi L   Xie Zhunyi Z   Liu Hongli H   Wan Ying Y   Wu Yuzhang Y  

Proceedings of the National Academy of Sciences of the United States of America 20090828 37


Antigen cross-presentation in dendritic cells is a complex intracellular membrane transport process, but the underlying molecular mechanisms remain to be thoroughly investigated. In this study, we examined the effect of siRNA-mediated knockdown of 57 Rab GTPases, the key regulators of membrane trafficking, on antigen cross-presentation. Twelve Rab GTPases were identified to be associated with antigen cross-presentation, and Rab3b/3c was indicated to be colocalized with MHC class I molecules at p  ...[more]

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