Ontology highlight
ABSTRACT:
SUBMITTER: Kim JS
PROVIDER: S-EPMC2747802 | biostudies-literature | 2009 Jul
REPOSITORIES: biostudies-literature
Kim Jason S JS Anderson George P GP Erickson Jeffrey S JS Golden Joel P JP Nasir Mansoor M Ligler Frances S FS
Analytical chemistry 20090701 13
A microfabricated flow cytometer was used to demonstrate multiplexed detection of bacteria and toxins using fluorescent coded microspheres. Antibody-coated microspheres bound biothreat targets in a sandwich immunoassay format. The microfluidic cytometer focused the microspheres in three dimensions within the laser interrogation region using passive groove structures to surround the sample stream with sheath fluid. Optical analysis at four different wavelengths identified the coded microspheres a ...[more]