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Synthetic substrate for application in both high and low throughput assays for botulinum neurotoxin B protease inhibitors.

ABSTRACT: A FRET peptide substrate was synthesized and evaluated for enzymatic cleavage by the BoNT/B light chain protease. The FRET substrate was found to be useful in both a high throughput assay to uncover initial 'hits' and a low throughput HPLC assay to determine kinetic parameters and modes of inhibition.

SUBMITTER: Salzameda NT

PROVIDER: S-EPMC2753755 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature

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Synthetic substrate for application in both high and low throughput assays for botulinum neurotoxin B protease inhibitors.

Salzameda Nicholas T NT Barbieri Joseph T JT Janda Kim D KD

Bioorganic & medicinal chemistry letters 20090827 20

A FRET peptide substrate was synthesized and evaluated for enzymatic cleavage by the BoNT/B light chain protease. The FRET substrate was found to be useful in both a high throughput assay to uncover initial 'hits' and a low throughput HPLC assay to determine kinetic parameters and modes of inhibition. ...[more]

PMID: 19747823

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