Ontology highlight
ABSTRACT:
SUBMITTER: Lewandowski JR
PROVIDER: S-EPMC2754755 | biostudies-literature | 2009 Apr
REPOSITORIES: biostudies-literature
Lewandowski Józef R JR De Paëpe Gaël G Eddy Matthew T MT Griffin Robert G RG
Journal of the American Chemical Society 20090401 16
We describe a new magic angle spinning (MAS) NMR experiment for obtaining (15)N-(15)N correlation spectra. The approach yields direct information about the secondary and tertiary structure of proteins, including identification of alpha-helical stretches and interstrand connectivity in antiparallel beta-sheets, which are of major interest for structural studies of membrane proteins and amyloid fibrils. The method, (15)N-(15)N proton assisted recoupling (PAR), relies on a second-order mechanism, t ...[more]