Ontology highlight
ABSTRACT:
SUBMITTER: Han H
PROVIDER: S-EPMC2759070 | biostudies-literature | 2009 Sep
REPOSITORIES: biostudies-literature
Han Hongling H McLuckey Scott A SA
Journal of the American Chemical Society 20090901 36
Primary amines present in protonated polypeptides can be covalently modified via gas-phase ion/ion reactions using bifunctional reagent ions. The use of reagent anions with a charge-bearing site that leads to strong interactions with the polypeptide, such as sulfonic acid, gives rise to the formation of a long-lived adduct. A distinct reactive functional group, an aldehyde in the present case, can then undergo reaction with the peptide. Collisional activation of the adduct ion formed from a reag ...[more]