Unknown

Dataset Information

0

Interleukin-10-induced MARCH1 mediates intracellular sequestration of MHC class II in monocytes.


ABSTRACT: IL-10 is a potent anti-inflammatory cytokine interfering with antigen presentation by inducing the intracellular sequestration of MHC class II (MHC-II) molecules. Here we studied the contribution of membrane-associated RING-CH (MARCH) ubiquitin ligase family members to the IL-10-induced down-regulation of MHC-II molecules. We found that MARCH1 and MARCH8 proteins are the most potent family members for the down-regulation of MHC-II surface expression in transfected cells, but only MARCH1 mRNA expression is strongly induced by IL-10 in human primary monocytes. We detected mono- and poly-ubiquitinated forms of MHC-II molecules both in IL-10-treated monocytes and in cells transfected with MARCH1. We also show direct interaction between MHC-II and MARCH1 molecules in co-immunoprecipitation assays. Finally, we found that siRNA-mediated knockdown of MARCH1 reverses IL-10-induced MHC-II down-regulation in primary monocytes. Thus, the immunosuppressive effect of IL-10 on antigen presentation is mediated through induced expression of MARCH1.

SUBMITTER: Thibodeau J 

PROVIDER: S-EPMC2759377 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature

altmetric image

Publications


IL-10 is a potent anti-inflammatory cytokine interfering with antigen presentation by inducing the intracellular sequestration of MHC class II (MHC-II) molecules. Here we studied the contribution of membrane-associated RING-CH (MARCH) ubiquitin ligase family members to the IL-10-induced down-regulation of MHC-II molecules. We found that MARCH1 and MARCH8 proteins are the most potent family members for the down-regulation of MHC-II surface expression in transfected cells, but only MARCH1 mRNA exp  ...[more]

Similar Datasets

| S-EPMC6042767 | biostudies-literature
| S-EPMC1142372 | biostudies-literature
2005-04-01 | GSE2435 | GEO
| S-EPMC3186872 | biostudies-literature
| PRJEB15527 | ENA
| S-EPMC5159193 | biostudies-literature
| S-EPMC7465434 | biostudies-literature
| S-EPMC6113260 | biostudies-literature
| S-EPMC3085167 | biostudies-literature
| S-EPMC5568224 | biostudies-literature