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Label-free and dynamic detection of biomolecular interactions for high-throughput microarray applications.

ABSTRACT: Direct monitoring of primary molecular-binding interactions without the need for secondary reactants would markedly simplify and expand applications of high-throughput label-free detection methods. A simple interferometric technique is presented that monitors the optical phase difference resulting from accumulated biomolecular mass. As an example, 50 spots for each of four proteins consisting of BSA, human serum albumin, rabbit IgG, and protein G were dynamically monitored as they captured corresponding antibodies. Dynamic measurements were made at 26 pg/mm(2) SD per spot and with a detectable concentration of 19 ng/ml. The presented method is particularly relevant for protein microarray analysis because it is label-free, simple, sensitive, and easily scales to high-throughput.

SUBMITTER: Ozkumur E

PROVIDER: S-EPMC2430348 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature

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Label-free and dynamic detection of biomolecular interactions for high-throughput microarray applications.

Ozkumur Emre E Needham James W JW Bergstein David A DA Gonzalez Rodrigo R Cabodi Mario M Gershoni Jonathan M JM Goldberg Bennett B BB Unlü M Selim MS

Proceedings of the National Academy of Sciences of the United States of America 20080603 23

Direct monitoring of primary molecular-binding interactions without the need for secondary reactants would markedly simplify and expand applications of high-throughput label-free detection methods. A simple interferometric technique is presented that monitors the optical phase difference resulting from accumulated biomolecular mass. As an example, 50 spots for each of four proteins consisting of BSA, human serum albumin, rabbit IgG, and protein G were dynamically monitored as they captured corre ...[more]

PMID: 18523019

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