Nucleosome deposition and DNA methylation at coding region boundaries.

ABSTRACT:

Background

Nucleosome deposition downstream of transcription initiation and DNA methylation in the gene body suggest that control of transcription elongation is a key aspect of epigenetic regulation.

Results

Here we report a genome-wide observation of distinct peaks of nucleosomes and methylation at both ends of a protein coding unit. Elongating polymerases tend to pause near both coding ends immediately upstream of the epigenetic peaks, causing a significant reduction in elongation efficiency. Conserved features in underlying protein coding sequences seem to dictate their evolutionary conservation across multiple species. The nucleosomal and methylation marks are commonly associated with high sequence-encoded DNA-bending propensity but differentially with CpG density. As th

SUBMITTER: Choi JK 

PROVIDER: S-EPMC2768978 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature